【Offline procurement consulting】

        Phone：15335258233；

        Email：engforlife-mkt@efl-tech.com

【product introduction】

      Silk fibroin ( SF ) is derived from the degumming of silk, and is a polypeptide composed of a variety of amino acids. SF molecule includes a hydrophobic peptide chain ( H chain ) and a hydrophilic peptide chain ( L chain ). The special amino acid sequences of H chain and L chain make it form a variety of protein secondary conformations. The properties of silk fibroin materials can be effectively controlled by regulating the secondary structure of silk fibroin, including the preparation of high strength and high orientation materials. SF has good biocompatibility, biodegradability and high tensile strength. It has been used in various biomedical fields, including wound dressings, artificial blood vessels, cell culture, etc.

      Silk fibroin methacryloyl ( SilMA ) is modified by methacryloyl modification of SF by glycidyl methacrylate to introduce double bonds on SF molecules. Due to the special spatial structure of SF molecule, it is easy to form crystallization and insoluble in water before modification. After the introduction of additional chemical groups, it can be quickly dissolved in water, which enables SilMA to be photocurable into hydrogels.

【Item】

【Applications】

      Cell culture, biological 3D printing, tissue engineering, etc.

【Storages】

      Dry kit: room temperature, 3 months; 4℃, 12 months; -20℃, 18 months.

【HNMR】

EFL-SilMA preparation process and HNMR

【Rheological characteristic】

The rheological properties of EFL-SilMA at different concentrations : ( A ) photocuring process ; ( B ) Storage modulus after curing ; ( C, D ) viscosity

【Compression characteristic】

Compression characteristics of EFL-SilMA with different concentrations (UV405nm,30mW/cm²,30s) : (A) stress-strain curve; (B) compression modulus 

【Biological properties】

Live-dead staining results of EFL-SilMA hydrogel cell culture

【3D printing performance】

SilMA gel structure printed by photocuring biological 3D printer

【Note】

       Silk fibroin solution is a semi-steady-state sol, which is easily induced by external stimuli such as strong shear, ultrasound, high temperature, organic solvents, etc., and molecular self-assembly occurs, precipitation or gelation behavior occurs. It is best to prepare it when using it. A small amount of insolubles in silk fibroin solution is normal. It is recommended to remove insolubles by centrifugation or filtration to avoid inducing more silk fibroin precipitation.

【Product Manual】-【EFL-SilMA】-Silk Fibroin Methacryloyl 

【Product Manual】-【EFL-SilMA (5g)】-Silk Fibroin Methacryloyl